Texas red-phalloidin
Web40 rows · A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized … WebThe three stains used for this BPAE cell were the Texas Red- Phalloidin (which made actin visible), the BODIPY FL stain (which made tubulin visible), and finally DAPI (which made the nucleus visible). The three excitation wavelengths used to examine the cells were UV (365 nm), yellow/greer (552 nm), and blue (495 nm).
Texas red-phalloidin
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WebJun 1, 2024 · Texas Red®-X phalloidin is a high-affinity F-actin probe conjugated to our bright, photostable, red fluorescent Texas Red®-X dye. Selectively stains F-actin. … WebPhalloidin, Alexa Fluor ™ Plus 647 Phalloidin, and Alexa Fluor Plus 750 Phalloidin. Note: For a complete list of fluorescent or biotinylated phalloidin conjugates, see Table 1 on page 5. Contents and storage Contents Cat. No. Amount[1] Storage[2] Fluorescent phalloidin See Table 1 on page 5. 1 vial of lyophilized solids (300 units/assay[3])
WebMay 20, 2024 · DAPI (1:2000) was applied for nuclear staining and Texas Red Phalloidin (1:400) was applied for the staining of actin at the same time as the secondary antibody. Cells were imaged and the colocalization of the 67LR with actin was analyzed on a Leica TCS SP8 confocal microscope with the same settings that were described in the previous … WebThe cell culture was simultaneously stained with Texas Red phalloidin and DAPI, which target the cytoskeletal actin network and DNA in the nucleus, respectively. The absorption maximum of BODIPY FL is 505 nanometers and the emission maximum occurs at 513 nanometers, while the corresponding values for Texas Red are 595 and 620 nanometers.
WebApr 12, 2024 · Actin was visualised with Texas-Red Phalloidin (Red). Scale bar is 2 µm (D) Average percentage of S. Tm associated with an actin-rich ruffle. WT or ∆N-WASP MEFs were pre-infected with unstained WT S. Tm (WT) or treated with PBS as a control (PBS), then infected for 5 minutes with WT S. Tm pre-stained with Alexa-Fluor 350. ... WebThe green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).
WebTexas Red X Phalloidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol …
WebApr 15, 2024 · Dr. Pepper is the closest thing Texas has to a state soda. The refreshing, fruity tanginess of that popular soft drink is world-famous, but it calls Waco home, where … crab and chicken gumboWebTag:TEXAS RED-X PHALLOIDIN Related Product Information According to the laws, regulations and policies related to " patent products ", the sale of this product is … district court of md hyattsville mdhttp://www.microscopyu.com/techniques/fluorescence/nikon-fluorescence-filter-sets/dual-band-excitation-filter-sets/dual-band-excitation-fitc-tritc district court of md prince george\u0027s countyWebTexas Red Operates as a conjugated fluorophore Phalloidin (toxin derived from mushrooms) - binds to actin filaments in the cell Not fluorescent Rhodamine binds to the phalloidin and operates as a rhodamine-phalloidin complex FIX Solution FIX solution contains paraformaldehyde district court of morgan countyWebExamine the fluorescence emission intensity from a culture of human lung cells ( MRC-5 line) stained with wheat germ agglutinin conjugated to Texas Red, Alexa Fluor 488 … district court of minnesota local rulesWebAdd Phalloidin-iFluor™ 594 Conjugate solution (100 μL/well) Stain the cells at room temperature for 20 to 90 minutes Wash the cells Examine the specimen under microscope with TRITC or Texas Red filter Important Warm the vial to room temperature and centrifuge briefly before opening. Storage and Handling Conditions crab and company wappapelloWebOct 1, 1999 · D, Primary-cultured mouse cerebellar granule neurons at 7 DIV were triple-stained with an anti-CPD polyclonal antibody (FITC;a), Texas Red-phalloidin (b), and an anti-synaptophysin monoclonal antibody (Cy5; c) and observed by confocal microscopy.d is a superimposed composite images ofa–c using three pseudocolors (green for CPD, red for ... district court of mobile county alabama