Webb1 maj 2024 · For the mouse cortex single nuclei RNA-seq data, Kallisto bus required 58.9 Gigabytes of memory, whereas STARsolo used 31.4 Gigabytes. The run time was similar. … Webb4 mars 2024 · At the same time STARsolo is ~10 times faster than the CellRanger. STARsolo is run the same way as normal STAR run, with addition of several STARsolo …
STARsolo: accurate, fast and versatile mapping/quantification
WebbBut as soon as I run STARsolo, I am facing a lot of issues. One of them ist, that despite of having two groups with each 300 cells, STARsolo creates a matrix.mtx where the read mapping is only taking place for the first cell! So all the other cells are excluded. command: WebbOur STARsolo repo but designed for Nextflow Tower. Contribute to cellgeni/nf-starsolo development by creating an account on GitHub. linearmotor 150mm
GitHub - dobinlab/STARsoloManuscript: Code for analyses in the …
Webb30 dec. 2024 · This study describes a novel single-cell RNA-seq technology called SCAN-seq which can capture the full-length transcripts in single cells based on the third-generation Nanopore sequencing platform, and demonstrates its performance on mouse preimplantation embryos. Webb11 mars 2024 · 鉴定新基因或转录本. ## 转录本拼装 使用STAR比对的结果拼装时,一定要加比对参数`--outSAMattrIHstart 0 --outSAMstrandField intronMotif`,不然出来的都是 … WebbSTAR source code and binaries can be downloaded from GitHub: named releases from https: //github.com/alexdobin/STAR/releases, or the master branch from … linear motion with stepper motor