Ovarian granulosa cell lines
WebNational Center for Biotechnology Information WebFeb 26, 2011 · Granulosa cell tumors of the ovary (GCT) are a specific subset of malignant ovarian tumors which, in contrast to the more common epithelial tumors, arise from the stromal cells of the ovary. GCT are the predominant form of ovarian stromal tumor, representing ~5% of all malignant ovarian tumors [ 1, 2 ].
Ovarian granulosa cell lines
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WebGranulosa cells in a woman’s ovaries play a key role in the female reproductive system. These cells release estrogen, progesterone and other hormones. Granulosa cells … WebApr 12, 2024 · This study investigated the roles of adiponectin in IVF treatment during Phase I (the basal stage before gonadotropin administration), Phase II (approximately 8 days after gonadotropin administration), and Phase III (on the ovum pick-up day), as well as the effects of adiponectin on CYP19A1 and the FSH receptor (FSHR) mRNA expression in a human …
Granulosa cell tumors (GCTs) are a type of ovarian tumor. They often produce hormones and lead to high estrogen levels. Women with a GCT may have irregular periods or bleeding after menopause. In young girls, a GCT may cause early puberty. When healthcare providers diagnose a GCT early, the outlook is … See more Experts don’t entirely understand what causes granulosa cell tumors. They often have a mutation (change) in the FOXL2 gene. FOXL2typically functions to help … See more The most common symptom of a GCT is high estrogen. In girls, high estrogen may cause early puberty. In adults, GCTs commonly cause: 1. Abnormal uterine … See more Without treatment, granulosa cell tumors can increase your risk of other health complications, including: 1. Endometrial hyperplasia, an unusually thick … See more WebFeb 18, 2024 · Cell lines and cell culture. The human ovarian granulosa cell lines KGN and COV434 kept in our laboratory with STR (short tandem repeat) identification were cultured in DMEM (HyClone, USA) supplemented with 1% fetal bovine serum (Gibco, USA) and 100 IU/mL penicillin–streptomycin at 37 °C in a humidified atmosphere of 5% CO 2 …
WebJun 1, 2024 · Since communication between ovarian cells has an important role, researchers have developed co-culture systems of follicular cells to elucidate the impact of chemical exposures on ovarian functions. Using a co-culture model of porcine theca cells and granulosa cells, Gregoraszczuk et al. [ 67 ]. studied the effects of BDE-47, BDE-99, … WebMar 30, 2024 · Thus, hormone-sensitive cancers, such as ovarian cancers, are exposed to these compounds. In the present study, we aimed to determine the effects of the concentrations of BPS and BPF found in body fluids on the cell viability, glucose uptake, glycolysis, oxygen consumption, and invasion by the adult ovarian granulosa cell tumor …
Web5 hours ago · In summary, in this study we identified target genes dynamically regulated by FOXL2 across ovarian development including known and newly identified FOXL2 …
WebMar 22, 2024 · FOXL2 and ESR2 are key transcriptional regulators in ovarian granulosa cells. To explore their transcriptional roles and their interplay, we have depleted Foxl2 and Esr2 in mouse primary granulosa cells to assess their ability to bind their targets and/or to modulate gene expression and cellular functions. We show that FOXL2 is involved in a … aiota full formWebDec 30, 2004 · Creation of appropriate in vitro cell model systems can provide important tools for the study of ovarian function. This has led to the development of ovarian … aiota conferenceWebJul 27, 2024 · Cell cultures. The human ovarian granulosa cell lines KGN and COV434 with STR (short tandem repeat) identification were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% fetal bovine serum ... a iotaWebEnter the email address you signed up with and we'll email you a reset link. aiota logoWebIn one model, ovarian granulosa cells isolated from 21-day-old female Sprague Dawley (SD) rats were cultured in vitro for 36 h and exposed to CdCl2 (0, 5, 10, and 20 μM), and in another model, a human ovarian granulosa tumor cell line (COV434) was used to construct the BIP- knockdown cell line sh-BIP and exposed to 0 and 20 μM CdCl2. after ... aiot digitimeWebFeb 25, 2024 · Granulosa cell tumours often produce hormones and are called functioning tumours. They make the female hormone oestrogen. This can cause symptoms such as … aiota incWebJun 19, 2024 · Western blot analysis was used to determine protein expression. RESULTS Results showed that the expression of miR-324 was aberrantly and significantly downregulated in PCOS ovarian tissues and KGN ovarian granulosa cells. Nonetheless, ectopic expression of miR-324 expression inhibited the viability of KGN cells via … a-iot