Methylphosphonate oligo
WebOligonucleotide-based therapies are a promising approach for treating a wide range of hard-to-treat diseases, particularly genetic and rare diseases. These therapies involve the use of short synthetic sequences of DNA or RNA that can modulate gene expression or inhibit proteins through various mechanisms. Despite the potential of these therapies, a … Web11 mei 1993 · A novel method of deprotecting methylphosphonate oligonucleotides was developed from these studies. The method incorporates a brief treatment with dilute ammonia for 30 minutes followed by addition of ethylenediamine for 6 hours at room temperature to complete deprotection in a one-pot format.
Methylphosphonate oligo
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WebThe present invention relates to an antisense oligonucleotide having SARS-CoV-2 translation control inhibiting activity. Targeting TIS-L within the leader sequence of SARS-CoV-2 gene, the antisense oligonucleotide of the present invention disturbs overall gene expression of SARS-CoV-2 to greatly reduce viral proliferation and infectivity and thus … Web26 jul. 2024 · Oligonucleotides have many important applications, including as primers in polymerase chain reactions and probes for DNA sequencing. They are proposed as a diagnostic and prognostic tool for various diseases and therapeutics in antisense therapy. Accordingly, it is necessary to develop liquid chromatography and solid phase extraction …
Web12 apr. 2012 · Methylphosphonate oligonucleotides carry a methyl group at their internucleotidic linkages. These internucleotide modifications were first proposed by Miller and Tso (1) and were specifically intended to address characteristics desirable for therapeutic use of oligonucleotides. Webused to solubilize the oligo. Methyl phosphonoamidites are deoxynucleoside amidites modified such that, when incorporated into an oligonucleotide, that base position will have a (electrically neutral) methyl phosphonate backbone linkage instead of the standard (negatively charged) phosphodiester linkage. Oligos containing one or more methyl ...
WebAddition of three carbon nucleophiles to Π, followed by stereospecific reduction, affords useful P-chiral phosphines; introduction instead of a single methyl group reveals the first stereospecific synthesis of methylphosphonate oligonucleotide precursors. WebAn Improved Method for the Synthesis and Deprotection of Methylphosphonate Oligonucleotides. Oligonucleotide Phosphorothioates. Synthesis and Purification of Phosphorodithioate DNA. Oligodeoxyribonucleotide Phosphotriesters. Oligonucleotide Boranophosphate (Borane Phosphate). Oligonucleotide Phosphorofluoridates and …
Web15 mei 2024 · These oligonucleotides, known as MethylPhosphonate Nucleic Acids (MePNA), when they contained various ratios of nucleoside-5′-phosphate and 5′- O -methyl-phosphonate units in an alternating mode exhibited superior enhancement of the RNase H …
WebAdditionally, the methylphosphonate linkage is resistant to nuclease hydrolysis. Here we show that a single methylphosphonate internucleotide linkage at the 3'-end of an oligo-2'-O-methylribonucleotide is sufficient to prevent degradation by the 3'-exonuclease activity found in mammalian serum. oxford iga nephropathyWeb1 dec. 1997 · A novel method of deprotecting methylphosphonate oligonucleotides with ethylenediamine was developed and found to be superior in yield of product by as much as 250%. 61 PDF Internucleotide phosphite sulfurization with tetraethylthiuram disulfide. Phosphorothioate oligonucleotide synthesis via phosphoramidite chemistry H. Vu, B. … oxford ifollowWebDevelopment of oligodeoxynucleotides (oligo-dNs) and their analogs as therapeutic agents is complicated by their low rate of transport across cellular membranes, which is required for interaction with the intracellular complementary nucleic acid sequences, and the lack of tissue-specific delivery. jeff lancaster hokWebThe disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Adenovirus nucleic acid. Compositions include amplification oligomers and/or detection probe oligomers. Kits and methods comprise at least one of these oligomers. oxford iga classificationWebwherein the double-stranded RNAi agent comprises a sense strand and an antisense strand forming a double stranded region, the antisense strand comprising a region of complementari oxford igcse ictWebA novel, structurally defined, and homogeneous oligodeoxynucleoside methylphosphonate (oligo-MP) neoglycopeptide conjugate, [YEE(ah-GalNAc)3]-SMCC-AET-pUmpT7, has been synthesized. The linkage between the carbohydrate ligand and the oligo-MP is a metabolically stable thioether. oxford igcseWeb1 jun. 1991 · Highly modified phosphorothioates or methylphosphonates are less stable than their partially modified counterparts which are less stable than the unmodified parent compounds. The 'normal' (2'-OH) RNA analog of duplex D1 is slightly destabilized, whereas the 2'-OCH3 RNA derivative is significantly stabilized relative to the unmodified DNA. jeff lamp wells fargo