Chip affinity
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Chip affinity
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WebMay 23, 2024 · We show that ProBound quantifies transcription factor (TF) behavior with models that predict binding affinity over a range exceeding that of previous resources; captures the impact of DNA... WebInteraction/affinity map of His 6-tagged A/G fusion protein with mono-NTA-derivatized carboxymethyl dextran (top) and XanTec’s poly-NTA sensor chip (bottom; NiHC1000M). Affinity increases from the upper-right corner to the lower-left corner.
WebBLI at the CMI Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. WebFor mammalian samples, numerous ChIP-grade antibodies are available that are validated for this procedure. For target proteins for which qualified antibodies are unavailable, fusion proteins such as HA, myc or GST can be expressed in the biological sample, and then antibodies against the affinity tags can be used to immunoprecipitate the target.
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